Journal: Pathology and Oncology Research

Article Title: GPNMB-Positive Cells in Head and Neck Squamous Cell Carcinoma—Their Roles in Cancer Stemness, Therapy Resistance, and Metastasis

doi: 10.3389/pore.2022.1610450

Figure Lengend Snippet: Immunohistochemical analysis of GPNMB, CSC markers, and EMT marker in serial sections of biopsy specimens. Serial sections of biopsy specimens were immunostained with the anti-GPNMB, anti-SOX2, anti-Nanog, and anti-Snail/Slug antibodies. Here demonstrated are two representative cases, one is an HNSCC expressing a high level of GPNMB (A) , the other is an HNSCC expressing a low level of GPNMB (B) . Note that the expression level of GPNMB is well correlated with those of CSC and EMT markers in serial sections of the same specimen. Magnification, ×200.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-GPNMB antibody (1:50, 20338-1-AP, Proteintech, Rosemont, IL, United States), rabbit monoclonal anti-SOX2 antibody (1:100, ab93689, Abcam, Cambridge, United Kingdom), rabbit monoclonal anti-Nanog antibody (1:300, ab109250, Abcam), and rabbit polyclonal anti-SNAIL + SLUG antibody (1:900, ab93689, Abcam).

Techniques: Immunohistochemical staining, Marker, Expressing

Journal: Oncology Reports

Article Title: Fangchinoline exerts antitumour activity by suppressing the EGFR-PI3K/AKT signalling pathway in colon adenocarcinoma

doi: 10.3892/or.2020.7857

Figure Lengend Snippet: FAN represses EMT and EMT regulatory factors in colon adenocarcinoma cells. (A and B) E-Cadherin, N-cadherin, vimentin, MMP2 and MMP9 expression following treatment with FAN (0–7 µM, 48 h) was determined by western blotting. (C and D) The expression of related transcription factors after FAN treatment (0–7 µM, 48 h) was measured by western blotting. *P<0.05 and **P<0.01 vs. 0 µM group. FAN, fangchinoline; EMT, epithelial-mesenchymal transition; SNAIL, zinc finger protein SNAI1; SLUG, zinc finger protein SNAI2; ZEB1, zinc finger E-box-binding homeobox 1.

Article Snippet: After the membranes were blocked with 5% non-fat milk in TBS with 0.1% Tween-20 for 1 h at room temperature, they were incubated overnight at 4°C with primary antibodies targeting the following proteins (as appropriate): Phosphorylated (p)-AKT (Ser473; polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 4060S; Cell Signaling Technology, Inc.), EGFR (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 18986-1-AP; ProteinTech Group, Inc.), E-cadherin (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 20874-1-AP; ProteinTech Group, Inc.), N-cadherin (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 22018-1-AP; ProteinTech Group, Inc.), vimentin (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 10366-1-AP; ProteinTech Group, Inc.), MMP2 (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 10373-2-AP; ProteinTech Group, Inc.), MMP9 (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 10375-2-AP; ProteinTech Group, Inc.), CD133 (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 66666-1-Ig; ProteinTech Group, Inc.), cyclin D1 (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 60186-1-Ig; ProteinTech Group, Inc.), Bax (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 50599-2-Ig; ProteinTech Group, Inc.), Bcl-2 (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 12789-1-AP; ProteinTech Group, Inc.), zinc finger E-box-binding homeobox 1 (ZEB1; polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 21544-1-AP; ProteinTech Group, Inc.), ZEB2 (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 14026-1-AP; ProteinTech Group, Inc.), zinc finger protein SNAI1 (SNAIL; polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 13099-1-AP; ProteinTech Group, Inc.), zinc finger protein SNAI2 (SLUG; polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 12129-1-AP; ProteinTech Group, Inc.), AKT (polyclonal, rabbit anti-human IgG; 1:1,000; cat. no. 10176-2-AP; ProteinTech Group, Inc.) and β-actin (polyclonal, mouse anti-human IgG; 1:1,000; cat. no. TA-09; OriGene Technologies, Inc.).

Techniques: Expressing, Western Blot, Binding Assay